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Introduction

From Sample Prep, HPLC/UHPLC to MS Instrumentation

Part I. Sample Preparation in LC-MS :

• The role/benefits of sample preparation in good LC-MS practice
• Overviews of different sample preparation techniques and their applicability
• Guide to selecting the right Sample Preparation method

Part II. Intro to Liquid Chromatography :

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Survey of different LC bonded phases and their application
• Guide to selecting the right LC column for your application

Part III. Introduction to LC-MS :
Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

• Atmospheric Pressure ​Ionisation theory, techniques and methods:
  • Desolvation
  • Electrospray Ionisation (ESI)
  • Atmospheric Chemical Ionisation (APCI)
•  Mass Analysis
  • The importance of calibration, and the differences between nominal mass, accurate mass and mass resolution
• Mass Analysers
  • Quadrupole – benefits, limitations and general usage
  • Time of Flight – benefits, limitations and general usage
• Data analysis
  • Quantitation
  • Application of targeted techniques eg. MRM to data acquisition and processing
  • Qualitative approaches to identification
  • Application of discovery techniques eg. scanning, information dependent acquisition, library searching

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Venue

The Principal Hotel, Manchester

The Principal Hotel, Manchester, Oxford St, Manchester, M60 7HA, United Kingdom

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Organised by

Contact information

Mark Comber
Phenomenex
Melville House,
Queens Avenue
Macclesfield
SK10 2BN
1625504070
Contact us by email

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