Exploring the Power of Eluent pH in LC Method Development
Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and
analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention
behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in
analyte retention from eluent pH can be helpful for screening systems and method development
platforms where observing the number of components in samples is important before method
optimisation is attempted. The chromatographic selectivity ‘space’ for various eluent and column
permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical
hints and tips for ensuring repeatable and reproducible chromatography, when working with an
extended pH range, will be included.
• Exploring chromatographic selectivity • Choosing appropriate stationary phases
• Stationary phase design • HPLC and UHPLC
• Acidic and basic analyte ionisation theory • Practical hints and tips
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
Eluent phase pH can be a powerful tool for the chromatographer. Choosing buffer systems and
analysis conditions that span an extended pH range (pH 2 - 11) can have an effect on the retention
behaviour and provide alternative selectivity for many acidic and basic analytes. Changes in
analyte retention from eluent pH can be helpful for screening systems and method development
platforms where observing the number of components in samples is important before method
optimisation is attempted. The chromatographic selectivity ‘space’ for various eluent and column
permutations will be described for a range of acidic, basic and neutral analyte mixtures and practical
hints and tips for ensuring repeatable and reproducible chromatography, when working with an
extended pH range, will be included.
• Exploring chromatographic selectivity • Choosing appropriate stationary phases
• Stationary phase design • HPLC and UHPLC
• Acidic and basic analyte ionisation theory • Practical hints and tips
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
